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The aim of this work was to study influence of the plasma treatment on hydrotalcite-type catalyst properties. Development of new catalysts for the petrochemical industry has high potential primarily in the field of extraction products from the biomass resources. Nonthermal atmospheric pressure dielectric barrier discharge (DBD) under defined conditions has been used for modification of catalysts. Hydrotalcites with Mg/Al molar ratio 3:1were prepared by coprecipitation. Catalysts were used either without treatment or after calcination or with subsequent plasma modification for both cases. Characterization of the catalysts was performed by techniques XPS, SEM, XRD, XRF and BET. Catalytic activity was tested using aldol condensation of furfural and acetone. The investigated catalysts showed different catalytic performance and the best catalyst was uncalcined plasma treated hydrotalcit.