The effect of addition of rejuvenators of various origins on bituminous binder and its properties was experimentally tested on bituminous binder, which was prepared by laboratory ageing to a level corresponding to the properties of the extracted binder from the R-material. The rejuvenators were added to fluxed binder to the grade 50/70. The evaluation of the original binder, laboratory aged binder and binder with the addition of rejuvenators included basic tests, rheological tests, resistance to ageing, and SARA group analysis.